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(12) United States Patent ao) Patent No.: us 6,264,915 Bi
Schiestl (45) Date of Patent: *Jul. 24,2001
FOREIGN PATENT DOCUMENTS
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(List continued on next page.)
Primary Examiner—Jill D. Martin
Assistant Examiner—Anne-Marie Baker
(74) Attorney, Agent, or Firm—Kohn & Associates
A process for screening an agent to determine its effect upon the frequency of genome rearrangement in transgenic mammals. The process comprises the steps of: (a) providing a transgenic mammal into which repeated genetic elements have been inserted into its haploid genome. The repeated genetic elements are sufficiently homologous so that, under ambient conditions, they recombine with each other and give rise to an identifiable genome rearrangement at a rate of at least about lxlO-11 occurrences per cell per generation. In a preferred embodiment the rearrangement can be identified as a phenotypic event or by PCR. The process further comprises (b) exposing at least one of the transgenic mammals to the agent to be tested, thereby providing an exposed mammal and (c) determining the extent of genome rearrangement which exists in a first exposed animal selected from the group consisting of the exposed mammal, its offspring, and mixtures thereof. The process then (d) compares the extent of genome rearrangement existing in the first animal with the extent of genome rearrangement existing in a second unexposed mammal and the offspring of the unexposed control mammals, and mixtures thereof.
1 Claim, 1 Drawing Sheet
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