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(12) Ullltfild States Patent (10) Patent No.: US 7,981,664 B1 Dultz (45) Date of Patent: Jul. 19, 2011 (54) APPARATUS AND METHOD FOR 5,446,534 A 3/ 1995 Goldman PERFORMING LIGAND BINDING ASSAYS 514831346 A 1/1996 Bum 5,485,277 A 1/1996 Foster ON MICROARRAYS IN MULTIWELL PLATES 5,491,097 A 2/1996 Ribi at al, 5,491,556 A 2/1996 Stewart et al. (75) Inventor: Shane C Dultz, Westlake Village, CA A ganning t 1 Us , , 3.IlSSOIl G 3 . ( ) 5,633,724 A 5/1997 King et al. (73) Assignee: Maven Technologies, LLC, Los g IS_I;1;l1,_l,It1(;gn et al Arigeles, CA (US) 5,738,825 A 4/1998 Rudigier et al. _ _ _ _ _ 5,753,518 A 5/1998 Karlsson ( * ) Notice: Subject to any disclaimer, the term of tlns (Continued) patent is extended or adjusted under 35 U-S-C l54(l>) by 500 days FOREIGN PATENT DOCUMENTS (21) Appl. No.: 12/125,685 AU 742417 _ 2/2°00 (Continued) (22) Filed: May 22’ 2008 OTHER PUBLICATIONS (51) Int CL Tadashi Saitoh, et al.“Optical Characterization of Very Thin HydroCIZM 3/00 (2006101) genated Amorphous Silicon Films Using Spectroscopic (52) U.S. Cl. ................ .. 435/287.2; 422/407; 422/82.11; Eiiipsomeii-ye, Japanese Journal OfApp1ied physics, V61, 31), N6, 435/288.4; 436/518; 436/524; 436/531; 436/164; 1113, Nov, 1991, pp, L1914-L1916, 436/805; 436/809 _ (58) Field of Classification Search ...................... .. None (commued) See application file for complete search history. Primary Examiner i Chris L Chin (56) References Cited (74) Attorney, Agent, or Firm — Haynes and Boone, LLP
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Each well of a multiwell plate adapted to accept a plurality of biological samples for testing for the presence of analytes in the sample is further adapted herein for immobilizing on the side walls of a well a 3D arrangement of ligand arrays A well is formed with planar side walls and a stack of sample separators is placed within a well in positions so as to not come in contact with ligand spots in the array(s). The spacing between each pair of adjacent separators in a stack forms a sample chamber in a position corresponding to an array of the 3D arrangement of ligand arrays.
20 Claims, 8 Drawing Sheets
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