Biosensor for analyzing quantitatively analyte with a predetermined size and ...

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US007332314B2

(12) United States Patent

Chang et al.

(io) Patent No.: (45) Date of Patent:

US 7,332,314 B2 Feb. 19, 2008

(54) BIOSENSOR FOR ANALYZING

QUANTITATIVELY ANALYTE WITH A
PREDETERMINED SIZE AND LARGER
THAN, AND MANUFACTURING METHOD
THEREOF

(75) Inventors: Je-Young Chang, Anyang-si (KR);

Seung-Joo Kang, Seoul (KR)

All Medicus Co., Ltd., Anyang-Si (KR)

(56)

(73) Assignee: ( * ) Notice:

Subject to any disclaimer, the term of this patent is extended or adjusted under 35 U.S.C. 154(b) by 0 days.

(21) Appl. No.: 10/558,222

(22) PCT Filed: May 21, 2004

(86) PCT No.: PCT/KR2004/001197

§ 371 (c)(1),

(2), (4) Date: Sep. 18, 2006

(87) PCT Pub. No.: WO2004/104569 PCT Pub. Date: Dec. 2, 2004

(65) Prior Publication Data

US 2007/0042440 Al Feb. 22, 2007

(30) Foreign Application Priority Data

May 23, 2003 (KR) 10-2003-0033068

(51) Int. CI.

C12N11/06 (2006.01)

(52) U.S. CI 435/181; 356/237.1; 428/412

(58) Field of Classification Search 435/7.9,

435/287.2,181; 250/559.27,559.45; 356/237.1,

356/600; 428/412 See application file for complete search history.

(Continued)
FOREIGN PATENT DOCUMENTS
KR 2003-0045490 6/2003

(Continued)

OTHER PUBLICATIONS

Korean Patent Abstracts, Publication No. 1020030045490, Jun. 11, 2003, 2 pp.

(Continued)

Primary Examiner—Long V. Le
Assistant Examiner—N Yang

(74) Attorney, Agent, or Firm—MacPherson Kwok Chen & Heid LLP

The present invention discloses a biosensor for quantitatively analyzing a bio-material and a manufacturing method thereof. The biosensor has an exposed conductive region of a few-nanometer scale distributed on an insulated metallic substrate in a desired pattern or randomly. The quantitative analysis of protein can be carried out by means of simplified procedures, without the necessity of rinsing out a signalproducing material, which is non-specifically bonded to the materials to be analyzed. The biosensor utilizes only the size of the molecules, and thus can be universally used for the analysis of bio-materials. A selective and separate analysis can be realized in which interference caused by other materials is significantly reduced.

7 Claims, 5 Drawing Sheets