System for monitoring cell motility in real-time

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(12) United States Patent

Kim et al.

(io) Patent No.: (45) Date of Patent:

US 7,033,819 B2 *Apr. 25, 2006

(54) SYSTEM FOR MONITORING CELL MOTILITY IN REAL-TIME

(75) Inventors: Enoch Kim, Boston, MA (US);

Gregory L. Kirk, Winchester, MA
(US); Olivier Schueller, Arlington, MA
(US); Emanuele Ostuni, Watertown,
MA (US)

(73) Assignee: Surface Logix, Inc., Brighton, MA
(US)

( * ) Notice: Subject to any disclaimer, the term of this patent is extended or adjusted under 35 U.S.C. 154(b) by 181 days.

This patent is subject to a terminal disclaimer.

(21) Appl. No.: 10/206,196

(22) Filed: Jul. 29, 2002

(65) Prior Publication Data

US 2003/0040031 Al Feb. 27, 2003

Related U.S. Application Data

(63) Continuation-in-part of application No. 09/709,776, filed on Nov. 8, 2000, now Pat. No. 6,699,665.

(60) Provisional application No. 60/374,799, filed on Apr. 24, 2002, provisional application No. 60/363,355, filed on Mar. 12, 2002, provisional application No. 60/334,548, filed on Dec. 3, 2001, provisional application No. 60/330,456, filed on Oct. 22, 2001, provisional application No. 60/328,103, filed on Oct. 11, 2001, provisional application No. 60/323,742, filed on Sep. 21, 2001, provisional application No. 60/307, 886, filed on Jul. 27, 2001.

(51) Int. CI.

C12M1/36 (2006.01)

(52) U.S. CI 435/286.2; 435/288.5;

435/288.7; 422/102

(58) Field of Classification Search 435/286.1,

435/288.7, 286.2, 288.5; 422/102 See application file for complete search history.

The invention relates to devices, devices for arraying biomolecules, including cells, methods for arraying biomolecules, assays for monitoring cellular movement, and systems for monitoring cellular movement. The devices include a support; a first layer configured to be placed in fluid-tight contact with the support, the first layer having an upper surface and defining a pattern of micro-orifices, each microorifice of the pattern of micro-orifices having walls and defining a micro-region on the support when the first layer is placed in fluid-tight contact with the support such that the walls of said each micro-orifice and the micro-region on the support together define a micro-well; and a second layer configured to be placed in fluid-tight contact with the upper surface of the first layer, the second layer defining a pattern of macro-orifices, each macro-orifice of the pattern of macro-orifices having walls and defining a macro-region when the first layer is placed in fluid-tight contact with the support and the second layer is placed in fluid-tight contact with the first layer such that the walls of the macro-orifice and the macro-region together define a macro-well.

6 Claims, 33 Drawing Sheets

190

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